木霉的RAPD分析

RAPD analysis of Trichoderma spp.

运用随机扩增多态性DNA(RAPD)技术对18个木霉菌株进行基因组DNA多态性分析。扩增结果表明,18个引物均反映出18个菌株间的遗传多态性,供试18个木霉菌株的RAPD分析与其生防效果之间不存在相关性。引物OPA-04与OPA-13对木霉菌全基因组DNA具有特征性分子标记。聚类分析结果表明,种间的聚类分析结果与形态鉴定的结果趋于一致。以欧氏距离5.0-5.5为适宜的阀值范围,RAPD遗传标记木霉菌可以作为该菌分类的有效手段之一。

Eighteen strains of Trichoderma spp. were analysised by method of random amplified polymorphic DNA (RAPD) with A-E group primers offered by Operon Corporation. Eighteen primers were screened out. The results of amplification showed obviously the diversity of the 18 isolates and no relationship was existed between the RAPD bands and the biocontrol effect of tested Trichoderma strains . The primers OPA-04 and OPA-13 have special characteristic molecular marker. UPGMA analysis of RAPD data showed that its result was consistent with those of identification by morphology. When euclidean distance was set at 5. 0~5. 5, RAPD genetic mark is one of the effective means of identification of Trichoderma.