HMW-GS组成不同小麦品种各种亚基形成和积累规律研究

Formation and accumulation of different HMW-GS in wheat

以3个HMW-GS组成不同的冬小麦品种为材料,研究了籽粒形成过程中亚基形成及积累表达规律。结果表明,不同亚基形成时间存在差异,Glu-D1、Glu-B1编码的亚基约在开花后第13天最早出现,Glu-A1编码的1亚基形成较晚。在灌浆期亚基已基本全部形成,但积累较慢;花后第20天后进入成熟过程,高分子量麦谷蛋白亚基大量迅速积累,开花第28～31天积累量达最高,之后含量有所下降。同一品种内不同亚基积累量不同,形成较早的Glu-D1、Glu-B1编码的亚基积累量最多;Glu-D1编码的X型亚基在所有供试品种中都有最多量的积累。

During grain formation of three common wheat cultivars from Shandong province,the subunits coded by Glu-D1 and Glu-B1 locus appeared firstly,at the time of 13 days after flowering;the subunit 1 coded by Glu-A1 appeared lastly.The forming time is different if the cultivars are different.During the forming time,all the subunits have been formed although the accumulation is not obvious.20 days after flowering,the accumulation begin to increase fast,and the accumulation arrives its peak at 28~31 days after flowering,then the accumulation begin to decrease.In the same cultivar,the different subunit accumulate differently.The accumulations of the subunits formed early are more than the subunits formed lately,and obviously the accumulation of X type subunit 5 or 2 coded by Glu-D1 is the biggest.