水稻强弱势籽粒核酸和蛋白质代谢差异

Difference of Nucleic Acid and Protein Metabolism in Superior and Inferior Grains of Rice

以两系杂交稻两优培九和培两优 5 0 0为试验材料 ,对强弱势粒核酸和蛋白质含量进行了测定。水稻抽穗当天 ,强势粒单位质量子房中总RNA、mRNA含量都高于弱势粒 ,蛋白质含量却低于弱势粒。抽穗后第 5天 ,强势粒单位质量子房中总RNA含量仍然高于弱势粒 ,mRNA含量两者差异不大 ,蛋白质含量依然低于弱势粒。不论是强势粒还是弱势粒 ,从抽穗到开花后第 5天 ,其总RNA、mRNA和蛋白质单粒含量都有显著的增加 ,且各时期强势粒都高于弱势粒。抽穗当天强势粒有两条特异蛋白质带出现 ,而弱势粒没有 ,抽穗后第 5天弱势粒才出现其中的一条。这说明强势粒与籽粒灌浆有关的基因表达比弱势粒早 。

By using two line hybrid rice Liangyoupeijiu and Peiliangyou 500, the RNA and protein contents in ovaries of superior and inferior grains were analyzed . At heading, total RNA contents per unit weight in superior grain ovaries were higher than those in inferior grain ovaries, and protein content contrarily. At the fifth day after heading, total RNA contents per unit weight in superior grains ovaries were still higher, the difference of the mRNA was not significant, protein contents per unit weight were lower compared with those in inferior grain ovaries. Either superior grains or inferior grains, the contents of total RNA, mRNA and protein in a single grain increased from heading to the fifth day after heading, and the contents in superior grains were higher than those in inferior grains at every stage. Two protein bands were found in superior grains but none in inferior grains at heading, and only one in inferior grains at the fifth day after heading. It suggested that the gene related to grains filling in superior grains expressed earlier and more briskly than that in inferior grains.