一种快速有效的741杨离体叶片再生芽方法(英文)

A new efficient method for rapid in-vitro shoot regeneration on leaf explants of Poplar 741

在对杂交品种 741杨 (Populusalba (P .davidiana×P .simonii)×P .tomentosa)进行农杆菌介导法转基因的试验中发现了一种快速有效的叶片再生芽的方法。首先叶片外植体在培养基I(MS培养基添加 0 .5mg/LBA和 1 .0mg/L 2 ,4 D)上培养 2～ 3d ,再转移到培养基SH(MSmediumcontaining 2 .0mg/LofBAand 0 .1mg/LofNAA)上培养 1 0d ,然后再转移到培养基II(MSmediumwith 0 .5mg/LofBA)上 ,培养大约 5d之后 86.7%的叶片外植体产生的芽 ,每片叶片外植体 ( 1cm× 1cm)可产生 40～ 5 0个芽。但是 ,如果叶片外植体在培养基I上培养的时间长于 5d ,再依次转移到培养基SH和II上 。

In the experiments of Agrobacterium tumefaciens-mediated transformation of the hybrid poplar 741, Populus alba (P. davidiana ×P. simonii) ×P. tomentosa, we developed a rapid and efficient method of shoot regeneration from the leaf explants in-vitro on Murashige and Skoog (MS) medium. The leaf explant segments was first cultured on medium I (MS medium supplemented with 0.5 mg/L of BA and 1.0 mg/L of 2,4-D ) for 2～3 days and then transferred to medium SH (MS medium containing 2.0 mg/L of BA and 0.1 mg/L of NAA) to culture for 10 days. 86.7% of leaf segments produced shoots after the leaf segments were transferred from medium SH to medium II (MS medium with 0.5 mg/L of BA) for 1 week. The average shooting number was 40～50 per 1 cm×1 cm of leaf segment. However, if the leaf explants incubated on medium I longer than 5 days, they produced more roots instead of shoots.