摘要
对目前南方大力推广的 18个葡萄品种进行了RAMP分析。结果表明 ,品种间遗传差异明显 ,多态性高。 33个引物组合产生的 14 8条DNA扩增片段中 ,12 0条具有多态性 ,占 81 1% ,每个引物组合可扩增 1～ 8条多态性带 ,平均 4 5条。遗传相似系数变化范围 0 75 9～ 0 94 5 ,平均值为 0 84 4。聚类分析显示 ,RAMP标记能将 18个品种完全分开 ,鉴定出引入栽培的京秀和信浓笑两个品种遗传关系很近。同时 ,RAMP标记的聚类结果与葡萄品种抗感霜霉病类型基本一致。
The RAMP analysis of the popular 18 grape breeds indicates that diversity genetic is present 33 primer combinations produce 148 bands, among which 120 (81.1%) are polymorphic. 1～8 polymorphic bands can be amplified from each primer combination, with an average being 4.5 bands. The genetic similarity coefficients range from 0.759 to 0.945, with the mean being 0.844. Cluster analysis shows that RAMP markers can distinguish the 18 breeds and identify the introduced Jing xiu and Shingno Smile resemblance of genetic relationships. Meanwhile, the cluster results of RAMP markers are almost identical with the anti-plasmopara uiticola types of grape breeds.
出处
《四川农业大学学报》
CSCD
2004年第2期133-137,共5页
Journal of Sichuan Agricultural University
基金
四川省科技厅"十五"育种攻关项目
四川省教育厅重点项目
关键词
葡萄
RAMP分析
品种鉴定
葡萄霜霉病
grape
RAMP analysis
greed identification
Plasmopara uiticola
