四川粗壮女贞苦丁茶中黄酮的提取、分离与初步鉴定

Extraction, Separation and Primary Identification of Flavonoids from Ligustrum robustum in Sichuan

以粗壮女贞 [Ligustrumrobustum (Rxob .)BL .]苦丁茶为材料 ,选用乙醇浸提L9(34)正交实验 ,确定了从四川苦丁茶中提取黄酮的最佳工艺条件 :乙醇浓度为 80 % ,提取温度为 80℃ ,乙醇用量为 10倍于苦丁茶质量 ,浸提时间为 4h ,总黄酮的提取效果最好 ,物质的浸出率可达 84 32 % ,所得干物质中黄酮总量为 4 7 6 80 3mg/ g。对提取物进行分离和纯化 ,得到 6种化合物 ,经薄层层析 (GF2 54)和显色反应等初步定性鉴定 ,结果表明 :化合物A为异黄酮 ;化合物B、D、F均为黄酮 (醇 ) ;化合物C为二氢黄酮 (醇 ) ;化合物E为花色甙。

By using Ligustrum robustum (Rxob.) BL. in Sichuan as materials and the experiment of L 9(34) by ethanol as extracting way, the optimum conditions of extracting flavonoids from Ligustrum robustum are obtained. When the condition is 80% of density of ethanol, 80、℃ of extracting temperature, 10∶1 ethanol volume and sample weight, 4、h of extracting time, the extracting effect is better, the extracting rate can reach 84.32% and the total content of flavonoids is 47.6803、mg/g. Then the extraction are separated and purified to gain six kinds of compounds. Primary identification of the structure by thin-layer chromatographic procedure of silica GF 254 and the color reactions show that compound A are isoflavones, compound B, D, F are all flavones (or flavonols), compound C are flavanones (or flavanonol), compound E are anthocyanins.