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一种新的用于删除选择标记基因的Cre/lox系统 被引量:2

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摘要 设计了一种新的诱导型Cre/lox似系统,并在转基因烟草(Mcofiana tabacum L.)中进行了验证。在诱导剂的作用下,位于同向lox位点之间的选择标记基因(hpt)和重组酶基因(Cre)在烟草愈伤组织中被删除。在该系统中,Cre基因在玉米乙酰苯胺类化合物诱导启动:(In5-2)的控制下表达。对转基因后代的分子检测结果表明,不论是否加入了诱导剂,目的基因(gus)均被整合到烟草基因组中;在诱导剂处理的48株转基因烟草T0代中,45株的hpt基因被删除了。该系统只使用一个载体,克服了二次转化系统带来的问题。
出处 《Acta Botanica Sinica》 CSCD 2004年第7期862-866,共5页 Acta Botanica Sinica(植物学报:英文版)
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