PDGF、TNF-α在人肝细粒棘球蚴囊壁周围组织的分层表达

Delaminated Expressions of PDGF and TNF-α in the Pericystic Tissues of Hepatic Hydatid Cysts

采用原位杂交方法检测血小板衍生生长因子 (PDGF )及肿瘤坏死因子α(TNF α )在 4 0例人肝细粒棘球蚴囊肿周围纤维囊壁中表达。结果显示PDGF、TNF α在肝细粒棘球蚴囊肿周围纤维囊壁中出现特异性分层表达。靠近虫体侧纤维囊壁中 ,PDGF、TNF α阳性细胞率分别为 (9 36 %± 2 13% )、 (10 5 2 %± 2 6 4 % )。靠近肝实质侧纤维囊壁中 ,PDGF、TNF α阳性细胞率分别为 (37 5 1%± 7 4 5 % )、 (2 5 76 %± 5 0 5 % )。PDGF、TNF α在两层中表达的均有显著性差异 (P <0 0 1)。同时 ,靠近肝实质侧纤维囊壁中PDGF与TNF α的表达也有显著性差异 (P <0 0 5 )。提示肝细粒棘球蚴囊肿周围人体纤维囊壁分层 ,PDGF、TNF α与肝实质侧纤维囊壁的形成有密切关系。

To observe the expression of PDGF and TNF-α in the pericystic tissues of hepatic hydatid cysts by means of in situ hybridization in 40 cases with hepatic hydatid,it was found that a special pattern of delaminated expression was observed in the pericystic layers of hepatic hydatid cysts. The percentages of PDGF and TNF-α positive cells in the pericystic layer near the worms were 9.36%±2.13% and 10.52%±2.64%,while those in the pericystic tissues near the hepatic parenchyma were 37.51%±7.45% and 25.76%±5.05% respectively. The difference of expression in these two layers was quite significant. Meanwhile,the expression of PDGF was higher than that of TNF-α in the pericystic tissues near the hepatic parenchyma. It suggests that PDGF and TNF-α might be involved in the formation of the pericystic layer near hepatic parenchyma.