苯接触工人外周血淋巴细胞染色体畸变分析

Chromosome aberration analysis of peripheral lymphocytes in workers exposed to benzene

目的 研究苯接触对工人外周血淋巴细胞染色体畸变的影响。方法 以个体采样器测定苯浓度 ,以非分带染色的方法分析外周血淋巴细胞染色体畸变。结果 苯接触组的数目畸变细胞率 [13 0 0 %(2 5 0 %～ 2 1 0 0 % ) ]高于对照组 (10 5 0 % (3 5 0 %～ 18 0 0 % ) ) (P <0 0 5 ) ,畸变细胞率 (不包括裂隙 ) [14 5 0 %(5 0 0 %～ 2 3 5 0 % ) ]高于对照组 [11 75 (3 5 0～ 18 0 0 ) ](P <0 0 5 ) ;超二倍体细胞率、数目畸变细胞率和畸变细胞率 (不包括裂隙 )与 8h TWA之间的剂量 效应关系的趋势检验的P值分别为 0 0 0 0 8、0 374 1和 0 2 816 ,与累积接苯浓度之间的剂量 效应关系的趋势检验的P值分别为 0 0 0 0 9、0 0 376和 0 0 4 86 ;苯接触组工人的未成熟着丝粒分离细胞率 [1 75 % (0～ 19 5 0 % ) ]与对照组 [1 0 0 % (0～ 15 0 0 % ) ]相比无显著性差异 (P >0 0 5 )。结论 苯接触导致外周血淋巴细胞染色体畸变增加 ,且呈剂量 -效应关系 ;苯接触是否诱发未成熟着丝粒分离有待进一步深入研究。

Objective To study the effects of benzene exposure on chromosome aberration of peripheral lymphocytes in workers.Methods Measure the benzene concentration with 3M passive dosimetry badges and analyze chromosome aberrations of peripheral lymphocytes using non-banding method. Results The numerically aberrated cell rate of the workers exposed by benzene [13.00%(2.50%-21.00%)] was significantly higher than that of the controls [10.50%(3.50%-18.00%)] (P<0.05), and the aberrated cell rate (excluded gaps) [14.50%(5.00%-23.50%)] was significantly higher than that of the controls [11.75(3.50-18.00)] (P<0.05), too; the P values of the dose-effect relationships between hyperploidy cell rate, numerically aberrated cell rate and aberrated cell rate (excluded gaps) and 8h-TWA were 0.0008, 0.3741 and 0.2816, respectively, and the P values of the dose-effect relationships between those and the cumulative exposure were 0.0009, 0.0376 and 0.0486, respectively; the premature centromere division cell rate of the benzene-exposed workers [1.75%(0-19.50%)] was not significantly higher than that of the controls [1.00%(0-15.00%)] (P>0.05). Conclusion Benzene exposure resulted in an increase of chromosome aberrations of the peripheral lymphocytes, which was in a dose-effect relationship manner; whether benzene exposure could induce premature centromere division deserves further studies.