受精鸡卵尿囊绒膜试验作为眼刺激试验替代方法的研究

Study on using the hen's egg test-chorioallantoic membrane as an alternative method of draize eye irritation test

目的 探讨受精鸡卵尿囊绒膜试验 (HET CAM)是否可作为体内眼刺激试验 (Draizetest)的一种替代方法。方法 用HET CAM的两种方法 ,即显微镜观察 (HET CAMscore)和台盼蓝染色法 (CAM TB)对 14种化学物的眼刺激性进行检测 ,并与Draizetest两种评分标准 ,即最大平均值 (MAS)和 2 4h作用值 (S2 4 )结果进行相关性比较。结果 HET CAMscore与MAS和S2 4的相关系数分别是 0 84 7和 0 779,CAM TB与MAS和S2 4分别是 0 86 2和 0 831。结论 HET CAM可用于替代Draizetest。

Objective To establish and study the hen's egg test-chorioallantoic membrane (HET-CAM) as an alternative method of Draize eye irritation test (Draize test). Methods 14 cosmetic ingredients were tested by the Hen's egg test-chorioallantoic membrane score (HET-CAM score) and the chorioallantoic membrane-trypan blue staining (CAM-TB) methods. Results Showed that compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and score of 24h after application (S24), the correlation coefficient between HET-CAM score and MAS or S24 was 0.847 or 0.779, while that between CAM-TB and MAS or S24 was 0.862 or 0.831 respectively. The results also showed that CAM-TB had a higher correlation with Draize test than HET-CAM score, partly because it is objective and quantitative. Also, the results showed that HET-CAM score had the greatest correlation with conjunctivae score of the three components, and so did the CAM-TB with corneal score. Conclusion It is suggested that the two types of HET-CAM can be used in a combined manner as an effective alternative method to Draize test.