香烟烟气溶液对大鼠淋巴细胞活力的影响及抗氧化物质的干预作用

Effect of cigarette smoke solution on viability of rat lymphocytes and the intervention of antioxidants

目的 了解香烟烟气溶液对大鼠淋巴细胞活力的影响 ,并探讨多种抗氧化物质的干预作用。方法以PBS作吸收液 ,用大包氏管收集香烟主流烟气 ,分别以 0、1× 10 - 3、2× 10 - 3、4× 10 - 3、8× 10 - 3、12× 10 - 3和16× 10 - 3支 毫升的香烟烟气溶液 (CSS)作用于大鼠淋巴细胞 ,2小时后加入AlamarBlue ,以其还原率评价细胞的活力。选择中作用剂量 8× 10 - 3支 毫升的香烟烟气溶液处理细胞 ,之前用维生素C(VC)、维生素E(VE)、谷胱甘肽 (GSH)、褪黑素 (MLT)、二甲基亚砜 (DMSO)等抗氧化物质预处理 ,检测细胞的AlamarBlue还原率。结果 2× 10 - 3～ 16× 10 - 3支 毫升各组AlamarBlue还原率均显著低于对照组 ,且随CSS浓度的增高而下降。 0 0 5mmol LVC、0 2mmol LVE、0 1mmol LMLT、1mmol LGSH、1%DMSO均能显著增加CSS染毒组细胞的AlamarBlue还原率。结论 CSS能使大鼠淋巴细胞活力下降 ,VC、VE、MLT、GSH、DMSO等抗氧化物质能拮抗这种损伤作用。

Objective To learn the effect of cigarette smoke solution(CSS) on viability of rat lymphocytes and the intervention of antioxidants. Methods CSS was prepared by bubbling main-stream cigarette smoke into PBS, then 0、1×10 -3cig/ml,2×10 -3cig/ml,4×10 -3cig/ml,8×10 -3cig/ml,12×10 -3cig/ml,16×10 -3cig/ml CSS were added into 7 groups of rat lymphocytes respectively. Alamar blue was added to each group 2 hours later, and after another 4 hours reduction rate of Alamar blue was detected and used to evaluate viability of cells. Otherwise, pretreated with vitamin C(VC), vitamin E(VE), glutathione(GSH), melatonin(MLT) or dimethyl sulfoxide(DMSO), cells were treated with 8×10 -3cig/ml CSS and reduction rates of Alamar blue were detected as before. Results Alamar blue reduction rates in 2×10 -3cig/ml～16×10 -3cig/ml groups were lower than that of control significantly, and there was a dose-effect relationship. Also, Alamar blue reduction rates increased in CSS-groups pretreated with 0.05mmol/LVC 、0.2mmol/LVE、0.1mmol/LMLT、1mmol/LGSH or 1%DMSO. Conclusion Viability of rat lymphocytes decreased in the presence of CSS, and it could be antagonised by antioxidants such as vitamin C, vitamin E, glutathione, melatonin and dimethyl sulfoxide.