黄芪多糖促进中性粒细胞与血管内皮细胞黏附及相关黏附分子表达

Effect of Astragalus Polysaccharides in Promoting Neutrophil-Vascular Endothelial Cell Adhesion and Expression of Related Adhesive Molecules

目的 观察黄芪多糖对中性粒细胞与血管内皮细胞黏附作用及相关黏附分子表达的影响,以探讨黄芪托毒生肌作用与其对伤口愈合中炎症反应作用的关系。方法 以黄芪多糖或黄芪多糖加白细胞介素1(IL-1)、肿瘤坏死因子(TNF)处理人中性粒细胞(PMN)或人脐静脉内皮细胞(HUVEC)后,用蛋白染料染色法研究黄芪多糖对PMN与HUVEC黏附的作用,用Cell-ELISA、APAAP法研究黄芪多糖对细胞表面黏附分子表达的影响。结果 黄芪多糖作用于HUVEC能明显促进其与PMN的黏附,而作用于PMN未见其作用增强。黄芪多糖与IL-1共同处理HUVEC,能促进IL-1诱导的PMN与HUVEC的黏附增强,且能增加由IL-1、TNF诱导的HUVEC表达黏附分子ICAM-1的表达;黄芪多糖处理PMN后对黏附分子CD18的表达无作用。结论 黄芪多糖可通过促进内皮细胞表面ICAM-1的表达而促进中性粒细胞与内皮细胞的黏附,促进伤口愈合中的炎症反应,这可能是黄芪托毒生肌作用的生物学基础之一。

To explore the detoxication and tissue generation effect of Astragalus (As) in wound healing and its relation with inflammatory reaction, through observing the effect of Astragalus polysaccharides (AP) on neutrophil-endothelial cell adhesion and expression of related adhesive molecules. Methods Human polymor-phonuclear leucocyte (PMN) or human umbilical vein endothelial cell (HUVEC) was treated separately with AP, AP plus interleukin 1 (IL-1) and tumor necrosis factor (TNF) to study the effect of AP on PMN adhesion with HUVEC by rose bengal staining, and that on expression of superficial adhesive factor by means of Cell-ELISA and APAAP method. Results When AP acted on HUVEC, it could significantly promote the adhesion of HUVEC with PMN, while when AP acted on PMN, the adhesion would not increase. When HUVEC was treated by AP plus IL-1, the IL-1 induced PMN adhesion with HUVEC could be strengthened, and the expression of HUVEC superficial adhesive factor ICAM-1 induced by IL-1 and TNF was strengthened also, but when PMN treated with AP, it showed no effect on the expression of adhesive factor CD18. Conclusion AP promotes the adhesion between neutrophil and endothelial cell by way of promoting the expression of superficial I-CAM-1 on surface of endothelial cells, so as to improve the inflammatory reaction in the wound healing course, it possibly is one of the biological bases of the detoxication and tissue generation effects of AP.