黏附分子在大鼠同种异体肝脏移植免疫反应中的作用

Expression of adhesion molecules in rat allografted liver

目的 探讨黏附分子ICAM 1、P selectin、E selectin、L selectin、PECAM 1和VCAM 1在肝脏移植中的表达及意义。方法 用免疫组织化学方法和原位杂交法 ,检测大鼠肝脏移植后不同时间 (1、3、5、7d)、不同模型中ICAM 1、P selectin、L selectin、E selectin蛋白、VCAM 1mRNA、PECAM 1mRNA的表达情况。结果 肝急性排斥组与自发耐受组相比 ,黏附分子ICAM 1、VCAM 1、PECAM 1、E selectin表达高 ;子代组与肝急性排斥组各指标表达类似 ,而ICAM 1的表达高于肝急性排斥组 ;半肝组与自发耐受组各指标表达类似 ,但ICAM 1、VCAM 1表达水平较自发耐受组高。P selectin、L selectin表达变化不明显。而正常大鼠肝脏未见黏附分子的表达。结论 肝排斥反应可能与黏附分子ICAM 1、VCAM 1、PECAM 1、E

Objective To investigate the effects of adhesion molecules including ICAM-1, VCAM-1, PECAM-1, P-selectin, L-selectin and E-selectin in rat liver allograft immune response in different stages. Methods After different rat allograft models were established in rats, ICAM-1, P-selectin, L-selectin and E-selectin were determined with immunohistochemistry. The expression of VCAM-1 mRNA and PECAM-1 mRNA was detected by in situ hybridization. Results Compared with liver allograft tolerance groups, the expression of adhesion molecules including ICAM-1, VCAM-1, PECAM-1 and E-selectin was enhanced in the acute liver allograft rejection group. The expression of ICAM-1 in F1 (P/D) D groups was higher than that in acute liver allograft rejection group. The expression of adhesion molecules detected in HLTx (P/2 D) group was similar with that in liver allograft tolerance group. There was no expression of the molecules in the normal rat liver. Conclusions Adhesion molecules including ICAM-1, VCAM-1, PECAM-1 and E-selectin may be involved in liver rejection response.