脑梗死及多梗死性痴呆患者血清S-100、NSE含量的测定

Assay of S-100 protein and neuron-specific enolase in blood of patients withcerebral infarction and multi-infarct dementia

目的 研究脑梗死 (CI)及多梗死性痴呆 (MID)患者血清S 1 0 0、神经元特异性烯醇化酶 (NSE)含量的变化及意义。方法 采用放免法及ELISA法对 72例脑梗死及 2 1例MID患者血清S 1 0 0、NSE含量进行测定。MID患者的智能水平采用简易智能量表 (MMSE)测定 ,并参考Hachinski缺血评分及DSM Ⅳ诊断标准进行确诊。结果 CI急性期患者血清S 1 0 0、NSE浓度明显高于对照组 (P <0 .0 1 ) ;而CI恢复期患者血清S 1 0 0、NSE的浓度与对照组无显著差异 (P >0 .0 5 )。MID患者血清S 1 0 0浓度高于对照组及CI恢复期组 (P <0 .0 5 ) ;而NSE浓度低于对照组及CI恢复期组 (P <0 .0 5 )。结论 血清中NSE及S 1 0 0蛋白的变化可反映CI及MID患者脑神经细胞。

Objective To study the changes of S 100 protein and neuron specific enolase (NSE)in blood and its significance in patients with cerebral infarction(CI)and multi infarct dementia (MID).Methods The concentration of S 100 protein and NSE in blood were determined in 72 patients with CI and 21 patients with MID by means of RIA and ELISA.Mini mental state examination (MMSE)was used to examine the intellectual level of MID patients,with Hachinski Ischemia Score and DSM IV diagnosis standard as references.Results S 100 protein and NSE concentration in blood in CI acute phase group were significantly higher than that in normal controls respectively( P <0.01);no significant difference was found in S 100 protein and NSE concentration in blood between CI recovery phase group and normal controls.S 100 protein concentration in MID patients was higher than that in normal controls and in CI recovery phase group( P <0.05);but NSE was lower than that in normal controls and in CI recovery phase group( P <0.05).Conclusion NSE and S 100 protein in blood could indicate the suffering situation and functional status of brain nerve cell and glial cell of patients with CI and MID.