HPLC法测定益气活血方药活性部位中五种蒽醌成分的含量

Quantitative Determination of 5 Kinds of Anthraquinone in the Active Fraction of Yiqihuoxue Formula by HPLC

目的 对益气活血方药活性部位中的芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行含量测定。方法 采用高效液相色谱法 ,Diamonsil C18分析柱 2 5 0mm× 4 6mm 5 μm ,流动相 :甲醇- 0 1%磷酸 (87∶13) ,检测波长 :4 36nm ,流速 :1 0ml min ,柱温 :室温。结果 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为 98 30 %、97 12 %、98 18%、97 6 6 %、97 16 %、RSD分别为 1 6 4%、1 15 %、0 95 %、1 5 1%、1 6 4% (n =5 )。结论 方法操作简便 ,准确 ,重现性好 ,可以用于该活性部位的质量分析。

Objective To determine the contents of aloe-emodin, rheic acid, emodin, chrysophanol, and emodin monomethyl ether in the active fraction of Yiqihuoxue Formula (YF). Method HPLC techniques were used in this experiment; the Diamonsil-C 18 column (250 mm×4.6 mm, 5 μm) was adopted, the mobile phase was methanol-0.1% phosporic acid (87∶13) at a flow speed of 1.0 mL/min, the detection wavelength was 436 nm, and the column was at the room temperature. Result The values of the average recovery of aloe-emodin, rheic acid, emodin, chrysophanol, and emodin monomethyl ether were 98.30%, 97.12%, 98.18%, 97.66% and 97.16%, respectively; and the values of RSD were 1.64%, 1.15%, 0.95%, 1.51% and 1.64%, (n=5.), respectively. Conclusion With a good repetition, this procedure is easy and accurate, and can be used in the analysis of the quality of the active fraction of YF.