活性肽在大鼠小肠吸收特点的实验研究

Effects of Soy Hydrolyzed Peptides Supplementation on Small Intestinal Absorption in Rats

目的 :研究补充活性肽对大鼠小肠上皮组织形态学、氨基肽酶活性及氮代谢的影响 ,探讨大鼠小肠对活性肽的吸收特点。方法 :雄性SD大鼠 30只 ,被随机分为 :安慰剂对照组 (Pla组 )、补充大豆分离蛋白对照组 (Pro组 )和补充活性肽实验组 (Pep组 )。大鼠在适应性喂养及膳食平衡 1周后 ,进行代谢实验及指标测试。结果 :(1)Pep组大鼠小肠粘膜蛋白含量比Pro组高 4 8 6 0 % ,比Pla组高 91 37% (P <0 0 5 )。 (2 )Pep组大鼠小肠粘膜氨基肽酶活性明显增加 ,比Pla组增加了2 0 3 97% ,比Pro组则增加了 73 30 % (P <0 0 5 )。 (3)Pep组大鼠小肠粘膜厚度比Pro组高 6 99% ,比Pla组高 2 5 0 3% (P <0 0 5 )。 (4 )Pep组大鼠小肠绒毛高度比Pro组高 4 6 5 % ,比Pla组高2 2 2 2 % (P <0 0 5 )。 (5 )Pep组大鼠小肠上皮隐窝深度比Pro组高 12 5 8% ,比Pla组高 35 77%。(6 )Pep组大鼠小肠上皮绒毛表面积比Pro组高 3 30 % ,比Pla组高 2 3 6 4% (P <0 0 5 )。 (7)补充大豆分离蛋白后 ,Pep组大鼠氮平衡值、氮储存率和表观消化率较Pla组分别增高 6 41 0 3%、5 91 4 2 %和 5 1 80 % ;而补充活性肽后 ,Pep组大鼠氮平衡值、氮储存率和表观消化率较Pla组分别增高10 6 1 5 4%、982 6 9%和 71 4 3% ,较Pro组分别增高 5 6

Objective: To investigate the effects of soy hydrolysed peptides supplementation on small intestinal morphology and nitrogen absorption in rats,and illustrate the characteristic of absorption on soy hydro? lysed peptides supplementation in small intestinal. Methods: 30 male SD rats were randomly divided into three groups: Placebo group (water supplemented, Pla group); Isolated soy protein-supplemented group (Pro group); Soy hydrolysed peptides-supplemented group (Pep group). After one week meals adaptation, Metabolic test had done, small intestinal morphology, and the activity of aminopeptidase were determined. Results: (2) The content of intestinal epithelial protein in Pep group increased 48.60% than Pro group, and increased 91.37% than Pla group (P<0.05). (2) The activity of aminopeptidase in Pep group increased 73.30% than Pro group, and increased 203.97% than Pla group (P< 0.05). (3)The mucosal thickness in Pep group increased 6.99% than Pro group, and increased 25.03% than Pla group (P<0.05). (4)The villous height in Pep group increased 4.65% than Pro group, and increased 22.22% than Pla group (P<0.05). (5) The crypt depth in Pep group increased 12.58% than Pro group, and increased 35.77% than Pla group (P<0.05). (6) The villous surface area in Pep group increased 3.30% than Pro group, and increased 23.64% than Pla group (P<0.05). (7)The value of nitrogen balance increased 641.03%, nitrogen retention rate increased 591.42%, and the apparent digestibility increased 51.80%, after isolated soy protein supplementation. Compared with Pla group, the value of nitrogen balance increased 1061.54%, the nitrogen retention rate increased 982.69%, and the apparent digestibility increased 71.43%, after soy hydrolysed peptides supplementation. Compared with Pro group, the value of nitrogen balance increased 56.75%, the nitrogen retention rate increased 56.59%, and the apparent digestibility increased 12.94%, after soy hydrolysed peptides supplementation. Conclusion: The small intestinal morphology and absorption improved, after soy hydrolysed peptides supplemented.