丹皮酚在体外对4种肿瘤细胞株的增殖抑制作用

Inhibitory effects of paeonol on the proliferation of four tumor cell lines

目的 探讨丹皮酚在体外对肿瘤细胞增殖的影响。方法 采用MTT法检测丹皮酚对体外培养的肿瘤细胞的增殖抑制作用。结果 丹皮酚在 7 81～ 2 5 0mg·L-1 剂量下对体外培养的人红白血病细胞系K562 、乳腺癌基因细胞系T61 7、肝癌细胞系Bel 74 0 4及宫颈癌细胞系HeLa的增殖均有抑制作用。药物浓度越高 ,抑制作用越强 ,有明显的剂量效应关系。对不同的细胞系抑制作用不同 ,其IC50 值分别为 :30 83(K562 )、1 3 4 4 (T6～ 1 7)、6 9 89(Bel 74 0 4 )及 93 91mg·L-1 (HeLa)。以丹皮酚 1 2 5及2 5 0mg·L-1 分别作用于以上细胞 ,于 2 4、4 8、72及 96h观察细胞的生长情况 ,发现丹皮酚对细胞的增殖抑制作用有明显的时间效应关系 ,作用时间越长 ,抑制作用越强。

AIM To study the infuence of paeonol on the proliferation of tumor cell lines in vitro. METHODS The inhibitory effect of paeonol on tumor cells proliferation was assayed by MTT dye reduction. RESULTS PAE at concentrations of 7 81～250 mg·L -1 inhibited proliferation of four tumor cell lines in vitro (human erythroleukemic cell line K 562 , breast cancer gene cell line T 6～17 , hepatocarcinoma cell line Bel 7404 and cervical cancer cell line HeLa) with different IC 50 (30 83, 13 44, 69 89 and 93 91 mg·L -1 , respectively). It was observed that higher concentration of PAE, more inhibitory effect, suggesting obvious concentration effect relationship of PAE. The inhibitory effects were augmented with the prolongation of culture time (24, 48, 72 to 96 h), suggesting time effect relationship of PAE. CONCULSION Paeonol may inhibit the proliferation of different types of tumor cell lines in vitro.