柱前衍生-HPLC法分析硫酸庆大霉素缓释片的释放度

Pre-column derivatization HPLC for determination of the release degree from gentamycin sulfate sustained-release tablets

以庚烷磺酸钠为反离子 ,利用庆大霉素结构中氨基同邻苯二醛 (OPA)、巯基醋酸在 pH 1 0 4的硼酸盐缓冲液中反应 ,生成 1 烷基 2 烷基硫代异吲哚衍生物 ,在 330nm波长处有强吸收 ,建立离子对色谱法对硫酸庆大霉素缓释片的释放度进行测定。较原UV法 ,柱前衍生 HPLC法影响因素少 ,重现性好 。

Taking heptane sulfonic acid sodium salt used as the counter ion, the amino groups in the structure of gentamycin sulfate structure react with o phthalaldehyde (OPA) and thioglycollic acid in a borate buffer solution (pH 10 4) to produce 1 alkyl 2 alkyl sulfo isoindoline derivatives showing a strong absorption peak around 330 nm which provide a ion pair chromatography method to determinate the release degree of gentamycin sulfate sustained release tablets. Compared with the original UV method, pre column HPLC method shows fewer influence factors and repeatable accuracy, which can effectively control the quality of gentamycin sulfate sustained release tablets.