米非司酮对大鼠异位子宫内膜超微结构影响

Effects of Mifepristone on Edometrium Ultrastructure in Rats with Endometriosis

目的 :探讨米非司酮对大鼠异位子宫内膜作用及超微结构影响。方法:建立WistarII级雌性大鼠子宫内膜异位症模型 ,实验组给予10(A组 ) ,25(B组 ) ,50(C组 )mg/(kg·d)米非司酮灌胃 ,对照组只给等量水灌胃 ,4周后处死动物。取异位结节、在位子宫进行病理学及超微病理学研究 ,取血测定雌激素、孕激素、皮质醇。结果:电镜下腺上皮微绒毛有处脱失而呈扁平状 ,整个细胞呈暗调 ,管腔内可见蛋白质分泌物 ,平滑肌细胞核淡染。血清雌、孕激素水平与对照组差别无统计学意义(P>0.05) ;C组血清皮质醇水平减低 ,与对照组差别有统计学意义(P<0.05) ,A组、B组与对照组相比差别无统计学意义(P>0.05)。结论:非米司酮可明显抑制大鼠异位内膜上皮及腺体 ,间质透明变性 ;影响到异位内膜结构。米非司酮对雌孕激素影响不明显。

Objective:To explore the effects of mifepristone on edometrium and ultrastructure in rats with endometriosis.Methods:Female endometrosis rat models with Wistar grade II were created by the gastric perˉfusion of10,25,and50mg/(kg.d)mifepristone respectively in experimental groups which included group A,B,and C,and water perfusion was performed in control group.All rats were killed4weeks after the perfuˉsion.Ectopic nodules and uterus on_position were removed for pathologic and ultrastructural patholgic examiˉnations,and the serum levels of estrogen,progesterone,and cortisol were examined respectively.Results:Elecˉtron microscopic findings showed that some of the microvillus of glandular epithelium were disappeared,the cell appeared darker,and the protein secretion was observed in the tract cavity.The smooth muscle nucleus was lightly stained.There was no significant difference in both serum estrogen and progesterone levels between exˉperimental group and control group(P>0.05).In comparison to control group,serum cortisol level decreased in group A(P<0.05),and there was no significant difference in serum cortisol between group A,B,and control group(P>0.05).Conclusion:Mifepristone can significantly inhibit endometriotic epithelium and glands,and reduce hyaline degeneratio in rats.It also can influence endometriotic structure and ultrastructural characterisˉtics.