去势雄兔干眼病模型角膜上皮细胞凋亡及相关基因表达的研究

Corneal epithelial cell apoptosis and related gene expression in castrated male rabbits with experimental xerophthalmia

目的 探讨去势雄兔干眼病模型角膜上皮细胞凋亡及相关基因表达与组织损伤的关系。方法 制作 6只去势雄兔干眼病模型 ,采用原位末端标记及免疫组织化学方法 ,对模型兔及 6只对照兔角膜上皮细胞中的凋亡细胞及Fas、FasL、Bax和bcl 2等相关基因蛋白的表达进行检测。结果 干眼病模型兔角膜上皮细胞凋亡数为 ( 5 2 3± 2 67)个 /高倍视野 ;对照兔为 ( 0 5 8± 0 13 )个 /高倍视野 (P <0 0 1)。模型兔角膜上皮细胞中 ,Fas、FasL及Bax阳性表达的细胞数均明显高于对照组 (P <0 0 1) ,与凋亡细胞数呈正相关 (r=0 78,0 82 ,0 76,P <0 0 5 ) ;bcl 2阳性表达的细胞数明显低于对照组 (P <0 0 1) ,与凋亡细胞数呈负相关 (r =-0 72 ,P <0 0 5 )。结论 去势雄兔角膜组织中上皮细胞凋亡可能是导致角膜变薄、干燥 ,进而功能丧失的原因之一 ;Fas、FasL及Bax的增加及bcl 2的减少均与促进细胞凋亡有关。

Objective To assess corneal epithelial cell apoptosis and related gene expression in castrated male rabbit with experimental xerophthalmia. Methods Six male rabbits were castrated,and experimental xerophthalmia models were established after 3 months.Small pieces of palpebral lobes of corneas were obtained from the models and normal controls.Apoptotic cells of corneas,expression of Fas,FasL,Bax and bcl-2 were detected using in situ end labeling and immunohistochemical staining. Results The numbers of apoptotic epithelial cells in corneas of xerophthalmia models were significantly higher than those in normal controls.Expressions of Fas,FasL and Bax were increased and expression of bcl-2 was decreased in epithelium cells in xerophthalmia models.There was a significant positive correlation between apoptotic cell numbers and the cell numbers that expressed Fas,FasL and Bax, and a negative correlation between apoptotic cells and bcl-2 expression. Conclusion The apoptosis may be one of the mechanisms leading to corneal cell death in xerophthalmia.The low androgen level can induce the apoptosis of cornea epithelial cells. Increased expression of Fas,FasL and Bax and decreased bcl-2 expression can promote apoptosis.