慢性前列腺炎患者精浆中热休克蛋白的表达及临床意义

Heat shock protein 70 expression in the seminal plasma of chronic prostatitis patients and its clinical significance

目的 探讨慢性前列腺炎患者精浆中热休克蛋白 70 (HSP 70 )的表达及临床意义。 方法 采用酶联免疫吸附测定法 (ELISA)对 10例慢性细菌性前列腺炎 (Ⅱ型 )、10例炎症型慢性非细菌性前列腺炎即炎症型慢性骨盆疼痛综合征 (ⅢA 型 )、10例非炎症型慢性非细菌性前列腺炎即非炎症型慢性骨盆疼痛综合征 (ⅢB 型 )患者及 6例健康志愿者精浆中HSP 70含量进行测定 ,并与慢性前列腺炎症状评分 (修订版 )进行相关性研究。 结果 Ⅱ型、ⅢA 型、ⅢB 型慢性前列腺炎患者及正常对照组精浆HSP 70含量分别为 (10 .74± 4 .0 2 )ng/ml、(2 .5 8± 0 .96 )ng/ml、(2 .78± 1.12 )ng/ml及 (6 .5 7±2 .72 )ng/ml。Ⅱ型患者精浆HSP 70含量显著高于正常对照组 ,P <0 .0 1,差异有显著性意义。ⅢA 型与ⅢB 型患者HSP 70含量均显著低于正常对照组 ,P <0 .0 1,差异有显著性意义。而ⅢA 型与ⅢB 型患者HSP 70含量间差异无显著性意义 ,P >0 .0 5。各组精浆HSP 70含量与慢性前列腺炎症状评分间均呈显著的负相关关系 (P <0 .0 1)。 结论 测定精浆中HSP 70的表达对各种类型慢性前列腺炎诊断有一定的临床应用价值 ,并可作为评价慢性前列腺炎病情程度的分子生物学指标。HSP 70在慢性前列腺炎的治疗方面也具有广阔的应用前景。

Objective To investigate the expression of heat shock protein 70 (HSP 70) in the seminal plasma of chronic prostatitis patients and assess its clinical significances in the diagnosis and treatment of chronic prostatitis. Methods The levels of HSP 70 in the seminal plasma of 10 patients with chronic bacterial prostatitis (typeⅡ),10 patients with inflammatory chronic pelvic pain syndrome (type Ⅲ_A),10 patients with non-inflammatory chronic pelvic pain syndrome (type Ⅲ_B) and 6 healthy volunteers were measured using enzyme-linked immunosorbent assay (ELISA).The results were analysed comparatively with chronic prostatitis symptom index (CPSI, modified version). Results HSP 70 levels in the seminal plasma were (10.74±4.02) ng/ml for patients with typeⅡ,(2.58±0.96)ng/ml for patients with type Ⅲ_A,(2.78±1.12)ng/ml for patients with type Ⅲ_B and (6.57±2.72)ng/ml for normal controls,respectively.The expression of controls was significantly lower than that of patients with typeⅡ( P <0.01) and was significantly higher than that of patients with type Ⅲ_A and type Ⅲ_B(both P <0.01).There was no significant difference of expression of HSP 70 between patients with type Ⅲ_A and type Ⅲ_B( P >0.05).The HSP 70 levels in the seminal plasma of all patients were correlated inversely with the CPSI ( P <0.01). Conclusions HSP 70 expression in the seminal plasma of chronic prostatitis patients provides a novel means for identification, characterization and potential management of the patients with chronic prostatitis.