鹿茸多肽对胎大鼠脑神经干细胞体外诱导分化的实验研究

EFFECT OF VELVET ANTLER POLYPEPTIDE(VAP) ON DIFFERENTIATION OF RAT BRAIN-DERIVED STEM CELLS IN VITRO

目的 探讨鹿茸多肽对胎大鼠脑神经干细胞体外分化的影响。 方法 从E12～ 14d的Wistar大鼠脑中分离扩增获得大量神经干细胞后 ,加入不同浓度的鹿茸多肽 ,观察其对胚胎神经干细胞分化的影响 ,并通过免疫组织化学染色检测神经干细胞分化为神经元和星形胶质细胞的状况。 结果 5 0 μg L组分化细胞总数与对照组相比有显著性差异 (P <0 0 1) ;5 0 μg L、10 0 μg L、2 0 0 μg L组神经元特异烯醇化酶 (NSE)阳性率与对照组相比有显著性差异 (P <0 0 1) ,并呈一定的剂量依赖性。 结论 鹿茸多肽在体外可明显促进神经干细胞向神经元分化 ,为鹿茸多肽应用于神经系统损伤性疾病的治疗提供了实验依据。

Objective To investigate the probabilities of brain-derived stem cells from fetal rats differentiating into neurons and astrocytes by velvet antler polypeptide(VAP) in vitro. Methods Neural stem cells from E12-14d rats were cultured for 7 days until neural stem cells (NSCs) aggregations were formed into neurospheres. The neurospheres were cultured at different concentrations of VAP, and immunocytochemistry was used to detect the differentiation of neural stem cells. Results The differentiated cells in 50μg/L VAP group are more than that in control group; the number of NSE positive cells in 50μg/L,100μg/L and 200μg/L groups is more than that in control group.Conclusion Neural stem cells can be successfully induced into neurons by VAP in vitro, which could provide a basis for regeneration of nerve system.;