人精子甘露糖受体表达与顶体反应的关系

INVESTIGATION OF RELATIONSHIP BETWEEN EXPRESSION OF MANNOSE-LIGAND RECEPTOR ON CAPACITATED HUMAN SPERM IN VITRO AND ACROSOME REACTION INDUCED BY SOLUBILIZED MOUSE ZONA PELLUCIDA

目的 研究人精子的甘露糖受体 (MR)表达与顶体反应的关系 ,以探讨体外获能培养精子的MR表达是否为人精子获能的标志。 方法 将上游法收获的活精子在获能培养基BWW中进行体外获能培养后 ,加入小鼠透明带溶解液 (mZPS)诱导人精子顶体反应 ,精子悬液用异硫氰酸荧光素标记的甘露糖基化牛血清白蛋白 (FITC DMA)为探针标记MR ,用豌豆凝集素 (PSA)法检测顶体反应。 结果 体外获能培养人精子的MR表达率与mZPS诱导的顶体反应率无相关性。 结论 体外获能培养精子的MR表达可能不是人精子获能的标志。

Objective To study the correlation between expression of mannose-ligand receptor(MR) on capacitated human sperm in vitro and acrosome reaction induced by zona pellucida(ZP). Methods The swimming-up sperms were incubated in capacitating medium BWW for 6 hours at 37℃, and then treated with solubilized mouse zona pellucida(mZPS).After an hour, the percentages of spermatozoa labeled with FITC-DMA were counted and used to show the expression of MR; the percentages of acrosome-reacted sperm visualized with fluoresceinated Pisum sativum agglutinin(PSA) were recorded. Results There is no correlation between expression of MR on capacitated human sperm in vitro and acrosome reaction induced by mZPS.Conclusion Expression of MR on capacitated human sperm in vitro might not be the essence of capacitation.;