中国大鲵(Andrias davidianus)7种组织器官蛋白水解酶的种类和活性分析

ANALYSIS OF PROTEASE KINDS AND ACTIVITIES IN SEVEN KINDS OF TISSUES AND ORGANS OF ANDRIAS DAVIDIANUS

目的 了解大鲵 (Andriasdavidianus)脑、心脏、肺、肾脏、眼球、皮肤和肌肉的蛋白水解酶种类和性质。方法 采用蛋白水解酶复性电泳 (G PAGE)技术。 结果 1 脑的蛋白水解酶在pH 4 5时有较弱的活性 ,在其他pH条件下没有活性 ;2 心脏和肺的蛋白水解酶在pH 4 5和pH 7 0时有活性 ;3 肾脏的蛋白水解酶在pH 4 5时有活性 ;在pH 7 0时 ,蛋白水解酶的活性强、种类多 ;4 在pH 4 5和pH 9 5条件下 ,未检出眼球有蛋白水解酶活性 ;5 肌肉的蛋白水解酶在pH 9 5时活性较强 ,在pH 7 0时 ,蛋白水解酶活性最强、种类最多 ;6 皮肤的蛋白水解酶种类与肌肉相似 ,但活性比肌肉弱。 结论 大鲵肾脏和肌肉的蛋白水解酶活性强、种类多 ;脑、心脏、肺、皮肤的蛋白水解酶活性弱、种类少 ;眼球几乎无蛋白水解酶活性。脑的蛋白水解酶活性的最适pH为酸性 ;心、肺和肾的蛋白水解酶活性的最适pH为中性偏酸 ;皮肤和肌肉的蛋白水解酶活性的最适pH为中性。

Objective Finding out the kinds and activities in protease of brain,heart,lung,kidney,eyes,skin and muscle of Andrias davidianus. Methods Using protease returned electrophoresis technique(G-PAGE). Results 1.Activity of proteases in brain was weak at pH 4.5,and showed no activity at pH 7.0 and pH 9.5;2.Proteases of heart and lung had activities at pH 4.5 and pH 7.0;3.Activity of kidney proteases was strong at pH 4.5 and stronger at pH 7.0,and kinds of proteases were more at pH 7.0 than at pH 4.5;4.Proteases in eyes had no activity at pH 4.5 and pH 9.5;5.Activity of proteases in muscle was very strong at pH 7.0 and pH 9.5,and kinds of proteases were more at pH 7.0 than at pH 9.5;6.Activity of skin proteases was similar to that of muscle,but the former was weaker than the later. Conclusions Among the seven kinds of tissues and organs of Andrias davidianus,kinds and activities of kidney and muscle proteases were stronger and more than others;and the eyes showed almost no protease activity.The proteases-activity optimal pH of brain was acidic;The proteases-activity optimal pH of heart,lung and kidney was acid of neutral;and the proteases-activity optimal pH of skin and muscle was neutral.