原位杂交与免疫组化技术在HPV检测中的应用

Comparison of In Situ Hybridzation and Immunohistochemistry Method Applied in HPV Detection

目的 :对两种HPV检测技术DNA原位杂交和免疫组化染色方法进行对比观察。方法 :选取本院外阴尖锐湿疣病例 95例 ,组织经固定、脱水、石蜡包埋、切片 ,分别采用原位杂交和免疫组化技术进行检测。结果 :HPV -DNA阳性率为 94 .7% (90 /95 ) ,阳性反应由鳞状上皮表层的空泡细胞核内延伸至棘层中下部近基底层的细胞核内 ;HPV -Ag阳性率为 6 3.1% (6 0 /95 ) ,阳性反应主要位于鳞状上皮表层的空泡细胞核内及角质层内 ,个别阳性细胞可在棘层出现。结论 :原位杂交技术在敏感度、阳性率及阳性强度、背景染色等方面均优于免疫组化方法 ,为尖锐湿疣的确诊及临床治疗提供更准确、可靠的依据。

Objective:To compare the detection efficiency of two technique: DNA In situ hybridzation and immunohistochemistry dyeing for detecting HDV. Method: 95 verified vulval condyloma acuminata samples were fixed, dehydrated, dew axed following standard protocols, and the sectioned slides were examined with both in situ hybridization and immunohistochemistry method. Result: Positive ratio of HPV-DNA is 94.8% (90/95)dectected by ISH. positive reaction extends from vacuole nucleus in surface layer of squamous epithelium to that of close basal layer in middle and lower part of stratum spinosum; Positive ratio of HPV-Ag is 63.1%(60/95)dectected by IHC. positive reaction is mainly in vacuole nucleus in surface layer of squamous epithelium and cuticular layer, a few exist in stratum spinosum. Conclusion: in situ hybridization is better than immunohistochemistry on sensitivity, positive ratio, positive intensity and background staining. It provides a more accurate and creliabte protocol for the diagnosis of HPV, thus ean in proveth and clinical treatment of condyloma acuminata.