人生长激素的酿酒酵母表达质粒构建的新方法

A New Method for Construction of Yeast Plasmid Expressing Human Growth Hormone

目的 :利用ECHO克隆系统建立一种更加快速、方便、通用的DNA重组技术。方法 :用常规重组法构建出ECHO系统中的携带外源基因HGH的供载体 ,再利用ECHO系统快速构建人生长激素的酿酒酵母表达载体 ,使HGH能在酿酒酵母启动子GAL的调控下进行转录。结果 :基因测序表明 ,在所构建的融合质粒载体上确实含有HGH的基因序列。结论 :本方法为研究HGH的消化道基因治疗打下基础。

ECHO cloning is a rapid,easy and common DNA recombination technique. In this study,the donor carrier for the foreign gene HGH was constructed by usual recombination,then the yeast carrier expressing HGH was constructed by ECHO system. As a result,HGH may be transcripted under the modulation of yeast promotor GAL.