摘要
To investigate the effect of arsenic trioxide (As2O3 )with three different concentration groups on the distribution and recruitment of dendritic cells(DCs) in the conducting airways of asthmatic mice. Methods: Fifty BALB/c mice were divided into 5 groups at random: control group, asthmatic group, therapeutic groups with low dose(1 mg/kg), moderate dose( 5 mg/lqg) and high dose( 10 mg/kg) of As2O3. The immunohistochomistry, scanning electron microscope and computerized image analysis were applied to detect airway DCs, respectively. Results: We demonstrated from the control mice that all intraepithelial NLDC-145 DCs throughout the respiratory tree cotdd be accounted for a network of cells with dendritic cell morphology, and the density of DCs varied from(500±50) cells/ram^2 epithelial surface in the large airways, to(60±10)cells/mm^2 epithelial surface in the small airways(P<0.05).The DCs characteristic of spiny and long processes were impressively observed by the scanning electron microscope. Comtmred with the control mlce,the density,but not the distribution of NLDC-145 DCs was significantly upregulated in the conducing airways of asthmatic mice(P<0.01) .Compared with the asthmatic mice, the densilies, but not the distributions of NLDC-145 DCs were significantly down regulated in the conducting airways of the thempeutic groups with three varying concentrations of As2O3(P<0.01 )while there were no statistical differences between each other(P>0.05).Conchsion : Our findings suggest that it might be an important therapeutic mechanism of As2 03 to downregulate not the distribution but the density of DCs in the conducting airways of asthmatic mice,and low dose of As203 has potential value in treating asthma.
Objective: To investigate the effect of arsenic trioxide(As_2O_3)with three different concentration groups on the distribution and recruitment of dendritic cells(DCs)in the conducting airways of asthmatic mice. Methods: Fifty BALB/c mice were divided into 5 groups at random:control group,asthmatic group,therapeutic groups with low dose(1 mg/kg),moderate dose(5 mg/kg)and high dose(10 mg/kg)of As_2O_3.The immunohistochemistry,scanning electron microscope and computerized image analysis were applied to detect airway DCs,respectively. Results: We demonstrated from the control mice that all intraepithelial NLDC-145 + DCs throughout the respiratory tree could be accounted for a network of cells with dendritic cell morphology,and the density of DCs varied from(500±50)cells/mm 2 epithelial surface in the large airways,to(60±10)cells/mm 2 epithelial surface in the small airways(P<0.05).The DCs characteristic of spiny and long processes were impressively observed by the scanning electron microscope.Compared with the control mice,the density,but not the distribution of NLDC-145 + DCs was significantly upregulated in the conducting airways of asthmatic mice(P<0.01).Compared with the asthmatic mice,the densities,but not the distributions of NLDC-145 + DCs were significantly down regulated in the conducting airways of the therapeutic groups with three varying concentrations of As_2O_3(P<0.01)while there were no statistical differences between each other(P>0.05). Conclusion: Our findings suggest that it might be an important therapeutic mechanism of As_2O_3 to downregulate not the distribution but the density of DCs in the conducting airways of asthmatic mice,and low dose of As_2O_3 has potential value in treating asthma.
基金
SupportedbygrantfromJiangsuProvincialAdministrationbureauofTCM(9974)
