摘要
Objective:To study the alteration of cyclin D1,p16 and pRB in glioma,analyze proliferation and apoptosis of tumor cells,and discuss the pathogenesis of glioma. Methods:Thirty-seven glioma specimens were classified as astrocytoma(25 cases,including 7 fibrillary cases;6 protoplasmic cases;12 anaplastic cases),and glioblastoma(12 cases,including 4 GBM cases).Ten normal brain tissues were taken as controls.The expression of cyclin D1,p16 and pRB were detected by immunohistochemical method.Cellular proliferation was assessed by Ki-67 label index(Ki-67 LI).Cellular apoptosis was detected by TUNEL and apoptotic indices(AI)was calculated. Results:The alterations of three proteins were cyclin D1 overexpression(28/37,75.7%),p16 and pRB deletion(20/37,54.1 % and 12/37,32.4%),which were closely related to tumor types,particularly in malignant glioma.Ki-67 LI and AI were higher when pRB pathway was abnormal.Apoptosis was minor in astrocytic tumors(astrocytomas,0.010±0.002;glioblastomas,0.057±0.016). Conclusion:The abnormalities of cyclin D1/p16-pRB pathway correlated closely with pathogenesis of glioma.
Objective:To study the alteration of cyclin D1,p16 and pRB in glioma,analyze proliferation and apoptosis of tumor cells,and discuss the pathogenesis of glioma. Methods:Thirty-seven glioma specimens were classified as astrocytoma(25 cases,including 7 fibrillary cases;6 protoplasmic cases;12 anaplastic cases),and glioblastoma(12 cases,including 4 GBM cases).Ten normal brain tissues were taken as controls.The expression of cyclin D1,p16 and pRB were detected by immunohistochemical method.Cellular proliferation was assessed by Ki-67 label index(Ki-67 LI).Cellular apoptosis was detected by TUNEL and apoptotic indices(AI)was calculated. Results:The alterations of three proteins were cyclin D1 overexpression(28/37,75.7%),p16 and pRB deletion(20/37,54.1 % and 12/37,32.4%),which were closely related to tumor types,particularly in malignant glioma.Ki-67 LI and AI were higher when pRB pathway was abnormal.Apoptosis was minor in astrocytic tumors(astrocytomas,0.010±0.002;glioblastomas,0.057±0.016). Conclusion:The abnormalities of cyclin D1/p16-pRB pathway correlated closely with pathogenesis of glioma.
基金
SupportedbygrantfromdepartmentofScienceandTechnology ,JiangsuProvince ,China(BS2 0 0 0 0 5 7)
