芦丁-α-鼠李糖苷酶分离提纯及其酶性质

Purification and characterization of rutin-α-rhanmosidase

报道了微生物sp.R9g产芦丁 α 鼠李糖苷酶的分离提纯及其酶性质鉴定。该酶能水解芦丁的一个 α 鼠李糖基,从而制备异槲皮甙。该酶蛋白的分子量约为72kDa,且酶反应的最佳温度是40℃,最适pH值是5。

This paper presents the separation and characterization of the rutin-α-rhamnosidase from microorganisms sp.R9g. This enzyme is capable of hydrolyzing the α-rhamnoside of rutin to produce isoquercitrin. Its molecular weight is 72 kDa. The optimum condition of enzyme reaction is 40?℃ and pH 5.