脑力苏胶囊改善VD大鼠认知障碍作用机制的实验研究

The Mechanism of Naolisu Capsule in Treating Vascular Dementia

目的 :通过脑力苏胶囊对血管性痴呆 (VD)大鼠学习记忆、海马乙酰胆碱酯酶 (AchE)和血浆生长抑素 (SS)含量影响的研究 ,探讨脑力苏胶囊改善VD大鼠认知障碍的机制。方法 :根据MassayaKato方法 ,采用双侧颈总动脉结扎法复制VD大鼠模型 ,实验大鼠随机分为假手术组、模型组、喜得镇组、脑力苏胶囊组。用水迷宫试验检测各组大鼠学习、记忆能力 ;借助放免法对海马AchE含量、血浆SS含量进行观测。结果 :喜得镇、脑力苏胶囊治疗后 ,大鼠学习记忆明显改善 (P <0 .0 5 ) ,海马AchE及血浆SS含量显著升高 (P <0 .0 0 1 )。脑力苏胶囊能显著改善VD大鼠学习记忆能力。其可能作用机制为增加海马AchE表达 ;

Objective:To study the mechanism of naolisu(NLS) capsule in treating vascular dementia(VD). Methods:Rat model of VD was established by durative ischemia and treated with NLS. Hydergine was taken for positive control. The content of plasma somatostatin(SS) and acetylcholinesterase(AchE) of hippocampus in rats were observed. Results: By comparison with sham operated group, the SS and AchE contents in model rats were increased significantly(P<0.01), and the learning and memory were improved, too. NLS showed an up-regulation on the above-mentioned parameters. Conclusion: NLS improves intellectual impairment of VD rats by increasing content of plasma SS and AchE of hippocampus, which in turn improve the neurotrophic effect and promote the restring function of injured neuron.