红豆草中单宁的分光光度法测定

Determining Tannin Level in Onobrychis spp. Used Spectrophotometry

本文筛选出乙醇溶液(乙醇:水=1:4)浸提红豆草中单宁,浸提率高;浸提液与FD(Folin—Denis)试剂显色,用分光光度法测定其含量,回收率达100.6±2.3％;用回归分析处理数据具有简单、准确、快速的优点。

Tannin in onobrychis spp. was extracted by alcohol solution (alcohol:water=1:4). When FD (Folin—Denis) reagent was added to extracted solution, reaction with colour produces. Determining tannin level used spectrophotometry. Recovery rate is 100.6卤2.3% Regresion lineal of analysis data was made, which is simple and accurate.