肝癌及癌旁组织中P^(53)基因第七外显子转录水平的表达差异及临床意义

Expression difference of the exon 7 of P^(53) gene on the level of transcription in hepatocellular carcinoma and its paracancerous tissues and its clinical significance

目的 :研究启东区肝癌中 P53基因第七外显子转录水平的表达及其与患者临床病理特征间的关系。方法 :通过 RT- PCR确定 P53基因第七外显子在 47对肝癌标本中的表达 ,并分析其与肝癌患者临床病理特征间的关系。结果 :P53基因第七外显子在肝癌及其癌旁组织中均显示差异表达 ,其中 1 7例为上调表达 ,30例为下调表达 ,上下调表达之间差异显著 ;女性、年龄≥ 45岁、AFP( + )、HBs Ag( + )、癌栓 ( + )、肝硬化、病理 Edmondson's 级的病例中 P53基因第七外显子均呈显著下调表达 ;Edmondson's I级病例肝癌组织中 P53基因的 EI显著高于 Edmondson's 级病例。结论 :启东肝癌中 P53基因第七外显子转录水平上存在表达差异 。

Objective :To investigate the expression difference of the exon 7 of P 53 gene on the level of transcription in hepatocellular carcinoma and its paracancerous tissues, and its association with clinicopathological characteristics. Methods:The expression difference was analysed in 47 pairs of hepatocellular carcinoma samples by RT-PCR. Results:There is a different expression of the exon 7 of P 53 gene between hepatocellular carcinoma and its paracarcinoma tissue in 47 liver cancer patients(100%,47/47). Of these patients, 17 cases showed exon 7 of P 53 gene up-regulation , and 30 cases showed exon 7 of P 53 gene down-regulation(P<0.05). Down-regulation expression rate of exon 7 of P 53 gene was higher than that of up-regulation in females;patients of over 45 years of age with AFP(+),HBsAg(+),tumor thrombus(+),cirrhotic liver,and Edmondson's III degree (P<0.05).The EI of Edmondson’s I grade patients is higher than that of Edmondson’s III grade ones. Conclusion: There is expression difference of the exon 7 of P 53 gene on the level of transcription in hepatocellular carcinoma from Qidong area, and its abnormal expression is associated with certain clinicopathological characteristics.