摘要
Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotech- nology. This review paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM). Precise manipulation has been realized including varied manipulating modes such as “cutting”, “pushing”, “folding”, “kneading”, “picking up”, “dipping”, etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.
Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotech- nology. This review paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM). Precise manipulation has been realized including varied manipulating modes such as “cutting”, “pushing”, “folding”, “kneading”, “picking up”, “dipping”, etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.
关键词
单DNA分子
纳米操纵
原子力显微镜
分子梳
分子切割
Nano-manipulation, Single DNA molecules, Atomic force microscopy, Molecular combing, Molecu- lar cutting
