趋化因子IP-10和MIP-3α在肝移植大鼠脾脏表达的研究

Expression of IP-10 and MIP-3α in rat spleen after liver transplantation

目的 检测趋化因子IP—10和MIP-3α在肝移植大鼠脾脏的表达来研究二者在肝移植免疫中的作用。方法 建立大鼠肝移植模型,在移植后不同的时间点(3、5、7、10d)取脾脏标本,用免疫组织化学法检测IP—10和MIP-3α在脾脏的表达。结果 IP-10和MIP-3α主要在动脉周围淋巴鞘、边缘区、红髓的淋巴细胞和巨噬细胞表达。二者均于移植后第5天表达达高峰,两组间表达有显著性差异。结论 IP-10和MIP-3α在脾脏的表达有助于DC-T细胞接触,从而活化T细胞,进而诱发特异性免疫应答。

Objective To explore the immune effects of IP-10 and MIP-3α in rat liver, we detect the expression of IP-10 and MIP-3α in spleen at different time postoperatively.Methods Different rat allografi models were established. Acute liver allografi rejection groups: Wistar liver to SD rats; Control groups: SD liver to SD rats. Spleen samples were collected on clays 3,5, 7,10 postoperatively (each n = 3) . The expressions of IP-10 and MIP-3α in spleen were examined by means of im-munohistochemistry. Results The expression of IP-10 and MIP-3α in spleen was mainly in lymphocytes and macrophages in periarterial lymphatic sheaths , marginal zone and red pulp. The expression of IP-10 and MIP-3α reached a peak on days 5 postoperatively and had significantly difference in spleen between acute rejection groups and control groups. Conclusions The high expression of IP-10 and MIP-3α in spleen may be favor of DC processing antigen to naive T cells and activating T lymphocytes .