急性胰腺炎细胞因子检测及Binder评分临床分析

A clinical analysis of the cytokine measurement and Binder point in acute pancreatitis

目的 了解细胞因子及器官功能和代谢合并症Binder评分在急性胰腺炎的变化从而早期识别重症急性胰腺炎(SAP)以利治疗。方法 应用化学发光免疫分析法检测83例急性胰腺炎及正常对照组30例的血清细胞因子IL6、IL8和TNF-α,应用酶联免疫吸附试验(ELISA)检测IL10的含量,结合Binder评分作出临床分析。结果 重症和轻症急性胰腺炎(MAP)细胞因子较正常对照组F明显增高(P<0.05),SAP组又较MAP组增高(P<0.05),Binder评分相应升高(P<0.01),差别有显著意义。结论 细胞因子检测结合Binder评分有助临床识别轻、重症急性胰腺炎且简捷易行。

Objective The cytokines and the Binder point based on the organic function failure and diabetic complications have been investigated in acute pancreatitis for early identifying and curing severe acute pancreatitis(SAP) . Methods Serum IL6, IL8 and TNF-α concentrations of 83 patients with acute pancreatitis and 30 normal controls were determined by the chemical luminous immunoanalysis; IL10 levels of serum were assayed by the double antibody sandwich ELISA method. Results It was found that the serum concentrations of IL 6,IL8,IL10 and TNF-α for the patients with both SAP and MAP (mild acute pancreatitis)were significantly higher than those of normal controls( P < 0.05) ,and the levels of these cytokines and the Binder point with SAP patients were rather higher than those with MAP patients( P < 0.01) . Conclusions The assay of cytokines and Binder point is helpful to distinguish mild or severe pancreatitis.