地龙2号抑制大鼠肝纤维化的研究

The study of Earthworm Ⅱ in preventing rat hepatic fibrosis induced by CC1_4

目的 研究地龙2号抑制大鼠肝内纤维组织形成的作用。方法 雄性Wistar大鼠,随机分成6组:正常组:不做药物处理;阴性对照组:皮下注射花生油,单灌蒸水;模型组:皮下注射CCl4,每日单蒸水灌胃;阳性对照组:造模方法同模型组,用秋水仙碱每日0.1 mg/kg灌胃;地龙大剂量组:造模同时用地龙2号每日50mg/kg灌胃;地龙小剂量组:造模同时用地龙2号每日25mg/kg灌胃。于8周末处死大鼠,取血测血清ALT、AST、AST/ALT,放射免疫法检测透明质酸(HA)和层黏连蛋白(LN),取肝组织作HE染色,病理分级。结果 地龙2号组与模型组相比,病理示肝纤维化程度明显减轻(P<0.05),肝细胞损害亦轻(AST/ALT大剂量组P<0.05,小剂量组P<0.01),且反应肝纤维化的指标HA及LN均显著降低(P<0.01)。结论 地龙2号有明显抑制大鼠肝纤维化形成的作用。

Objective To investigate the effect of Earthworm Ⅱ(Ew) on preventing the formation of hepatic fibrosis in rats. Methods Fifty-two male Wistar rats were randomly divided into six groups: normal group (N group, 6 rats), normal control group (NC group, 6 rats) , model group (M group, 10 rats), positive control group (PC group, 10 rats) , high dose of Earthworm Ⅱ group (Ew h group, 10 rats) and low dose of Earthworm Ⅱ group (Ew 1 group, 10 rats) .The rats of M, PC , Ew h and Ew 1 group were subcutaneously injected with CC14 for 8 weeks to induce liver fibrosis, and the rats in the NC group were injected with peanut oil. PC group rats were administrated colchicine and Ew h and Ew 1 group rats were administrated different dose of Earthworm Ⅱ. At the end of 8 weeks all rats were killed, The serum biochemical and fibrosis markers and the pathological grade of liver fibrosis were observed. Results The rank of hepatic fibrosis in animals of Ew h and Ew 1 group was significantly lower and the serum markers of HA,LN and AST/ALT also reduced obviously than those in model group ( P < 0.05 or P < 0.01) . Conclusion Ew can inhibit obviously the formation of liver fibrosis induced by CC14 in rats.