垂体肿瘤转化基因蛋白的表达与垂体腺瘤侵袭性和增殖能力的关系

Expression of pituitary tumor transforming gene(PTTG) protein and relations to cell proliferation invasion in pituitary adenomas

目的探讨人垂体腺瘤中垂体肿瘤转化基因(PTTG)蛋白的表达与肿瘤侵袭性和增殖能力的关系。方法采用免疫组织化学染色方法检测手术切除的63例垂体腺瘤(侵袭组45例,非侵袭组18例)组织中PTTG蛋白的表达,检测染色增殖细胞核抗原(PCNA),同时计数组织内微血管数量(MVD)。结果PTTG在侵袭性垂体腺瘤中的表达水平显著高于非侵袭性垂体腺瘤。侵袭性垂体腺瘤中PCNA标记指数和微血管密度也显著性高于非侵袭性垂体腺瘤。相关分析显示:PTTG表达与垂体腺瘤内PCNA标记指数和微血管密度呈正相关(P<0.05)。结论PTTG在垂体腺瘤形成过程中起重要作用,并与垂体腺瘤的侵袭性和增殖程度密切相关。

Objective To investigate the role of pituitary tumor transforming gene (PTTG) protein on tumor invasiveness and cell proliferation in human pituitary adenomas. Methods Immunohistochemical techniques were used to detect the expression of pituitary tumor transforming gene (PTTG) protein in 63 cases of surgical removed and paraffin-embedded pituitary adenomas (45 cases of invasive, 18 cases of non-invasive tumors). A polyclonal antibody against human Factor Ⅷ related antigen were used to identify microvascular density(MVD) and a monoclonal antibody against proliferating cell nuclear antigen (PCNA) was used as a marker for proliferating. Results PTTG protein increased significantly in invasive pituitary adenomas group than in non-invasive group. PCNA labelling index and MVD in invasive group were also significantly higher than non-invasive group. The expression of PTTG protein was positively correlated to PCNA labelling index and MVD. Conclusions PTTG may play important roles in early pituitary tumoregenesis and correlate with its invasiveness and cell proliferation.