杨尺蠖核多角体病毒的研究 Ⅵ.病毒的贮藏效果

STUDIES ON THE NUCLEAR POLYHEDROSIS VIRUS OF THE POPLAR LOOPER APOCHEIMA CINERARIUS Ⅵ. STORAGE EFFECT TEST ON THE VIRULENCE OF ACINPV

杨尺蠖核多角体病毒,在不同条件和不同时间的贮藏效果试验证明,在4℃冰箱或-20℃低温冰箱内贮藏,多角体制备物及其配制的AciNPV杀虫剂,其活性至少可保持两年;在室温条件下贮藏,多角体制备物的活性仅能保持一年,而加工制剂则可保持近两年。

The present paper reports the processing techiniques of the formulation of nuclear polyhedrosis virus of Apocheima cinerarius, and its detection of safety. New formulation of the AciNPV were made by incorporating the preparation of polyhedrals inclusion bodies (PIB) with sunlight protectant and other abjuvants in a blend screen of electroshak. Bioassays tests indicated that samples of the aceton-precipitation and new formulations of virus were very effective for early third-instar larvae. The LC50 and 95% fiducial limits were 2.84×103PIB/ml, 2.84 ×103PIB/ml, and 1.70×103 -4.76× 103 PIB/ml and 1.43×103-5.66×103PIB/ml respectively.Several powder formulations were directly diluted with water and mineral oil (NO. 0 diesel oil) when used in the field. The tests conducted on small plots and large blocks showed that several formulations of AciNPV were effective for the poplar looper 2-3 instar larvae in forest field. The mortality of poplar looper larvae was over 85% in 12-15 days.Safety tests showed that formulation of AciNPV was not hamper to domestic animals and did not couse mutation. It was safety for man. Bacteriological detection proved that pathogenic bacteria, such as Escherichia coli, Salmonell, Vibric, and Clostridium were cultured without isolation.