摘要
对比了两种不同的脱蛋白方法(酶+Sevage法和三氯醋酸法)在螺旋藻多糖提取工艺中的应用。研究结果表明,不同的除蛋白方法不仅影响蛋白质的去除率和多糖的得率,而且所得到的螺旋藻多糖在组分、分子大小及单糖组成上存在较大差异。
Two different de-protein processes (Enzymolysis+Sevage's method and Trichloroacetic acid method (TCA )) wereproposed to improve the traditional process for extraction of polysaccharide from Spirulina Platensis. The production rate of crudepolysaccharides (ESPE, TSPS, SPS), the contents of polysaccharides and the remained protein ratio in the crude polysaccharides,etc, were compared in this paper. ESPS and TSPS were further purified by loading on DE-52 and Sephadex - 100 . Twopolysaccharide fractions of ESPS and three fractions of TSPS were obtained. The molecular weights of these fractions were 96,200、85800, 64300, 37400, 17400 respectively. The different efficacy of different molecular weight polysaccharides shouldbe studied further.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第6期116-119,共4页
Food Science
