木耳多糖提取工艺研究

Studies on the Extracting Technology of Polysaccharide from Auricularia

本文讨论了黑木耳多糖的提取方法,实验结果表明,采用酶法可显著提高多糖提取率,其最佳提取条件为:料水比1:60,浸提温度80℃,浸提时间2.5h,中性蛋白酶用量650IU/g,作用温度43℃,作用时间1.5h,最适pH值7.4;纤维素酶用量375IU/g,作用温度43℃,作用时间1.5h,最适pH值5.3。木耳多糖提取率为4.38%。

The research on the extracting technology of Auricularia polysaccharide was studied in this paper. The results showedthat the extracting rate of polysaccharide was 4.38%.The extracting rate could be increased by enzymatic technology. The besttechnological conditions for the extraction were as follows: the ratio of material and water was 1:60 with a temperature of 80℃and extracting time 2.5h and the amount of Proteinase and cellulase used was 650 IU/g and 375 IU/g respectively with a reactingtime of 1.5h at the temperature of 43℃. The optimum pH of the two respectived enzymes was 7.4 and 5.3.