头孢他啶与胃蛋白酶相互作用的研究

Investigation on Interaction of Ceftazidime with Pepsin

采用荧光光谱和紫外吸收光谱法研究了头孢他啶与胃蛋白酶的荧光猝灭作用。结果表明,头孢他啶可以有规律地使胃蛋白酶内源荧光猝灭,其猝灭机理可认为是头孢他啶与胃蛋白酶形成复合物的静态猝灭,实验获得了不同温度下头孢他啶与胃蛋白酶作用的结合常数和热力学参数。根据所得结果可推断头孢他啶与胃蛋白酶的作用力为静电引力,同时由Förster非辐射能量转移理论计算得出了头孢他啶与胃蛋白酶结合位置的距离。

The interaction between Ceftazidime with pepsin and the mechanism of fluorescence quench were studied by fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. The results showed that the fluorescence of pepsin is quenched regularly by Ceftazidime. The binding constants and thermodynamic parameters of Ceftazidime with pepsin are obtained at different temperatures. The electrostatic interaction force plays a main role in the binding of Ceftazidime with pepsin. The distances of binding site between Ceftazidime with pepsin are also obtained according to the theory of Förster’s non-radiative energy transfer.