摘要
采用循环伏安法(CV),将β-环糊精(β-CD)电聚合在玻碳电极(GCE)表面,制得聚β-CD修饰玻碳电极(pβ-CD/GCE)。基于β-环糊精和尿酸(UA)之间的主客体识别作用,pβ-CD/GCE能有效地富集UA。用方波伏安法(SWV)考察修饰电极对UA的氧化信号。结果表明,UA的氧化峰电流明显增强;UA和抗坏血酸(AA)的氧化峰电位分离,从而能排除高浓度AA的干扰,实现对UA高灵敏、高选择性测定。在优化的实验条件下,该传感器对UA的检测表现出了宽的线性范围(10 μM^500 μM)、低的检测下限(0.54 μM,3倍信噪比)、良好的选择性和重复性,并可用于实际尿样的检测中。
The Poly-β-cyclodextrin modified glassy carbon electrode(pβ-CD/GCE)was prepared by electro-polymerization ofβ-cyclodextrin(β-CD)on the surface of glassy carbon electrode(GCE)by cyclic voltammetry(CV).Based on the host-guest interaction betweenβ-cyclodextrin and uric acid(UA),the pβ-CD/GCE could effectively enrich UA.The oxidation signal of UA at the modified electrode was determined by square wave voltammetry(SWV).The results showed that the oxidation peak current of UA was significantly enhanced;the oxidation peak potentials of UA and ascorbic acid(AA)were separated,demonstrating that the modified electrode could eliminate the interference of high concentration of AA,so as to realize the highly sensitive and selective determination of UA.Under the optimized experimental conditions,this sensor for UA showed wide linear range(10μM-500μM),low detection limit(0.54μM,S/N=3),and good selectivity and repeatability,and was applied in urine samples.
出处
《分析化学进展》
2019年第2期71-78,共8页
Advances in Analytical Chemistry
