酶–化学法羟基十八碳二烯脂肪酸玉米油的制备与测定

Preparation and Determination of Hydroxyoctadecadiene Fatty Acid Glycerides by Enzymatic Chemical Method

开发了一种绿色、温和的合成十八碳二烯脂肪酸玉米油的酶–化学方法。以大豆脂氧酶好氧催化氧化玉米油所得的氢过氧化玉米油为原料,以乙醇/乙酸乙酯稀释氢过氧化玉米油酯,与溶有亚硫酸钠水相混合,还原得到十八碳二烯脂肪酸甘油酯。考察了溶剂浓度、还原剂用量、温度、时间对氢过氧化玉米油还原产率的影响。最佳工艺条件为:15℃,以90% (V/V体系)的乙醇为溶剂,n(Na2SO3):n(-OOH) = 1.2:1、反应1.5 h,氢过氧化大豆油的转化率达91%。

A green and mild enzymatic chemical method for the synthesis of octadecadiene fatty acid corn oil was developed. The hydroperoxide obtained by aerobic catalytic oxidation of corn oil by soybean lipoxygenase was used as raw material. Corn oil hydroperoxide was diluted with ethanol/ethyl acetate and mixed with water dissolved in sodium sulfite to obtain octadecadiene fatty acid glyceride. Then sodium sulfite was dissolved in aqueous phase, the corn oil hydroperoxide was diluted with ethyl acetate/ethanol, and the octadecadiene fatty acid glyceride was obtained by reduction reaction. The effects of solvent concentration, reductant dosage, temperature and time on the reduction yield of hydroperoxide corn oil were investigated. The optimum process conditions were as follows: 15˚C, 90% (V/V system) ethanol as solvent, n(Na2SO3):n(-OOH) = 1.2:1, reaction for 1.5 h, and the conversion of soybean oil hydroperoxide reached 91%.