印记基因Igf2r和RB-1在代谢综合征不孕患者子宫内膜表达水平的研究

Expression of Imprinted Genes Igf2r and RB-1 in the Endometrium of Infertile Women with Metabolic Syndrome

目的:研究印记基因Igf2r和RB-1在代谢综合征(MS)不孕患者子宫内膜的表达。方法:选择2011年7月至2011年12月因不孕症来我院生殖中心就诊的MS患者11例作为实验组,代谢正常女性15例作为对照组,收集分泌期的子宫内膜组织,采用实时定量聚合酶链反应方法(RT-PCR)测定子宫内膜组织Igf2r和RB-1的表达水平。结果:Igf2r和RB-1在MS患者和代谢正常妇女子宫内膜中均有表达,与正常女性相比较,MS患者子宫内膜Igf2r表达水平下降(P = 0.0051),RB-1表达水平升高(P = 0.0364),具有显著性差异。结论:MS不孕患者子宫内膜组织印记基因Igf2r表达水平明显下降,RB-1表达水平明显升高,两者可能影响MS患者的子宫内膜容受性。

Objective: To investigate expression of imprinted genes Igf2r, RB-1 inthe endometrium of infertile women with metabolic syndrome (MS). Methods: From Jul. 2011 to Dec.2011 inour center, 11 infecund women with metabolic abnormalities were experimental group, a matched group of 15 normal women was choosed. Collected their prolife rative phase endometrial tissue, using real-time quantitative polymerase chain reaction (RT-PCR) to measure the ex- pressions of Igf2r and Rb-1 inthe endometrium. Results: Igf2r and RB-1 were both expressed in the endometrium of MS and normal women. Compared with the normal women, the expression of Igf2r in the endometrium of MS was decreased (P = 0.0051), while the expression of RB-1 was incresed (P = 0.0364), significant differences were observed between experimental group and matched group. Conclusions: The expression of imprinted gene Igf2r in the endo- metrium of infertile women with MS was decreased significantly, while the expression of RB-1 was increased, the 2 imprinted genes may impair the function of endometrium of MS.