摘要
目的:观察钙敏感受体(CaSR)表达变化对实验性糖尿病大鼠心肌超微结构的影响。方法:Wistar大鼠随机分为对照组、糖尿病4周组和糖尿病8周组。通过高糖高脂饮食喂养联合腹腔注射STZ (30 mg/kg)建立糖尿病模型。通过透射电镜观察各组大鼠心肌超微结构的改变,心肌组织CaSR蛋白的表达通过Western blot检测。结果:与对照组相比,糖尿病组大鼠心肌CaSR蛋白表达随着病程的延长逐渐降低,心肌结构损伤严重。结论:在大鼠糖尿病心肌病发生发展过程中CaSR的表达逐渐下降,可能导致糖尿病心肌损伤、发生糖尿病心肌病的原因之一。
Objective: To observe the effect of calcium sensitive receptor (CaSR) expression on myocardial ul-trastructure in experimental diabetic rats. Methods: Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups. Rats in the diabetes group were fed a high-glucose and high-fat diet, and intraperitoneal injection of streptozotocin (STZ, 30 mg/kg) to establish a type 2 diabetes model. Cardiac morphological changes were observed by electron microscope, and CaSR protein expression in cardiac tissue was detected by Western blot. Results: Compared with the control group, diabetic groups showed decreased expression of CaSR protein in the myocardium of diabetic rats, and gradually worsened with the prolongation of the course of the disease. Conclusion: The CaSR expression of myocardium is reduced in the progression of diabetes, and it may lead to changes in myocardial ultrastructure.
出处
《临床医学进展》
2019年第2期116-120,共5页
Advances in Clinical Medicine
基金
黑龙江省教育厅科技研究项目资助(12541276).
