摘要
目的:探讨中药茯苓及复方制剂对铝致老年痴呆症(AD)小鼠治疗效果的观察及对乙酰胆碱转移酶(chAT),乙酰胆碱酯酶(AchE)的影响。方法:建立铝致AD小鼠动物模型,将50只小鼠分为正常组、模型组、治疗1组和治疗2组;除正常组外,造模组用D-半乳糖 + AlCl3混合水溶液腹腔注射(Al3+ + 浓度2 mg/ml的三氯化铝水溶液按5 mg/kg体重剂量给小鼠稀释后腹腔注射60天)。治疗1、2组在铝染毒2个月后同时给予中药复方制剂不同剂量灌胃,模型组和正常组用等量体积蒸馏水灌胃,直至实验结束。在实验前、后分别进行水迷宫游水试验,并测定血红蛋白;实验结束后取血,分离血清,测定血清生化指标;处死小鼠取脑,称脑重量,制成脑匀浆,离心取上清,分别测定脑中乙酰胆碱酯酶(AchE)、乙酰胆碱转移酶(chAT)、超氧阴离子自由基()清除率、谷胱甘肽等含量;另部分脑用甲醛处理后作病理检查。结果:大脑(AchE)活力,正常组、模型组、治1组、治2组依次为,5.77 ±1.52▲、6.02 ±0.79、7.30 ±0.59、5.27 ±1.09▲▲ (U/mg.prot ),与治疗1组比较,▲P ▲▲P ★、7.05 ±5.07▲、52.95 ±25.79▲★★、53.95 ±12.82▲★★ (U/g组织湿重),与正常组比较,▲P ★P ★★P ▲▲、44.71 ±2.21★★、55.41 ±2.10★▲▲、41.30 ±3.36★★▲▽▽ (U/ml),与正常组比较,★P ★★P ▲P ▲▲P ▽▽P 3+含量依次为135.00 ±8.37、149.40 ±0.89、147.43 ±4.83、118.75 ±6.41 (ng/ml),超氧阴离子自由基()清除率依次为27.65 ±4.81、14.71 ±3.60、22.65 ±8.67、21.57 ±6.14 (%),造模前、中、后,Hb依次为正常组无明显变化、模型组降低、治疗组先降低后升高。结论:铝可导致大脑chAT下降、AchE活性升高,乙酰胆碱减少,胆碱能神经障碍,认知功能障碍,可能是老年痴呆症发病的重要原因,同时铝又使动物抗氧化能力降低,加重疾病;本中药复方制剂通过排铝、恢复(chAT) (AchE)酶活力,提高抗氧化能力,治疗后对老年痴呆症有明显疗效。而茯苓(治1组)比复方制剂疗效较差。
Objective:To explore the effect of Chinese herbal medicine and compound preparation on mice of aluminum-induced Alzheimer's disease(AD).Methods:AD mice model was established first.50 mice were divided into four groups:control group,model group,treatment group 1 and treatment group 2.Except the control group mice,the model group mice were injected intraperitoneally with mixed liquid of D-galactose+AlCl3 for 60 days(the mice were injected intraperitoneally with Al3+concentration of 2 mg/ml aluminum trichloride diluted liquid at a dose of 5 mg/kg body weight for 60 days).Treatment group 1 and treatment group 2 were poisoned for 2 months with aluminum first,then the two treatment groups were poured with different dosages of Chinese herbal compound preparations respectively;the same volume of distilled water was given to mice of the control group and model group till to the end of the experiment.Hemoglobin and Morris water maze test were tested before and after experiment.At the end of experiment,the blood was ob-tained,the serum was isolated,and serum biochemical indexes were measured.After mice sacri-ficed,their brains were taken and weighed,then the brains were made into homogenate,and cen-trifuged to get the supernatant,in which acetylcholinesterase(AchE),acetylcholine transferase(chAT),superoxide anion free radical()cleaning rate,and glutathione were determined.And another part of the brain was used for pathological examination after formaldehyde treatment.Results:In all experimental groups,the indices showed respectively that vitality of mice’s brain(AchE)was 5.77±1.52,6.02±0.79,7.30±0.59,5.27±1.09(U/mg.prot),P 3+in mice’s brain content turned out 135.00±8.37,149.40±0.89,147.43±4.83,118.75±6.41(ng/ml);superoxide anion free radical()cleaning rate was 27.65±4.81,14.71±3.60,22.65±8.67,21.57±6.14(%).Before,during,after the contamination,it showed that Hb in the control group had no obvious change,in model group was low,and in the treatment group was lower first then increased.Conclusion:Aluminum can induce the brain neuron cell suffering from atrophy,degeneration and necrosis,which will reduce the AchE activity,chAT activities,and the anti-oxidative ability.Chinese medicine compound preparations have obvious curative effect on AD through excreting aluminum and improving anti-oxidative ability.
出处
《临床医学进展》
2019年第6期807-814,共8页
Advances in Clinical Medicine
