脐带间充质干细胞对高原脑水肿大鼠水通道蛋白-4的影响

The Effect of UC-MSCs on Aquaporins-4 Expression in High Altitude Cerebral Edema in Rats

[目的]探讨间充质干细胞对高原脑水肿大鼠水通道蛋白4表达的影响。[方法]成功制作大鼠高原缺氧损伤模型8只,对照组及干细胞组各8只,测脑组织含水量,采用RT-PCR、免疫组织化学、Western Blot测定脑组织高原缺氧损伤24 h后水通道蛋白4 mRNA和蛋白表达变及间充质干细胞对其影响。[结果]高原缺氧损伤后,脑组织含水量增加,水肿明显,水通道蛋白4 mRNA和蛋白表达量上调(P 【0.05),应用间充质干细胞可有效抑制水通道蛋白4的表达。[结论]水通道蛋白4在高原缺氧脑水肿的形成过程中可能发挥了重要作用,应用间充质干细胞可有效抑制水通道蛋白4的表达,减轻高原脑水肿损伤。

[Objective] To study the relationship between AQP4 and high altitude cerebral edema and effect of UC-MSCs. [Methods] The model of high altitude cerebral edema was established (N = 8). Brain edema was measured. The expression of AQP4 mRNA and protein were measured by RT-PCR, immunohistochemistry and Western Blot and to detect the effect of UC-MSCs in on Aquaporins-4 expression in high altitude cerebral edema in rats. [Results] The water content increased and the edema was evident. The expression of AQP4 mRNA and protein were up-regulated (P