关于颅内动脉瘤破裂潜在基因的生物信息学挖掘

Bioinformatics Excavation of Potential Genes for Intracranial Aneurysm Rupture

目的:挖掘颅内动脉瘤(Intracranial Aneurysms, IAs)中的破裂颅内动脉瘤和未破裂动脉瘤之间的差异是基因并分析其在颅内动脉瘤破裂的潜在意义。方法:我们从GEO数据库获得了三个基因表达的数据集(GSE26969,GSE15629和GSE6551)进行差异基因分析,利用GO和KEGG分析并获得其中有意义的基因,利用Cytoscape中的分子复杂性模块(MCODE)插件寻找关键模块中的关键基因。结果:利用从GEO数据库获得的数据集进行多数据集分析后得出116个差异基因,56个高表达基因,60个低表达基因,进行蛋白表达网络构建后,利用Cytoscape中的分子复杂性模块(MCODE)插件寻找出关键基因为PTPRB。结论:经过生物信息学分析不同的颅内动脉瘤壁样本表明,PTPRB下调在颅内动脉瘤出现破裂具有较高的相关性,这为相关药物治疗并预防颅内动脉瘤破裂提供潜在的可选择靶点。

Objective: To excavate the difference between ruptured and unruptured intracranial aneurysms (IAs) for genetic analysis of its potential significance in the rupture of intracranial aneurysms. Methods: Three gene expression datasets (GSE26969, GSE15629 and GSE6551) were obtained from the GEO database for differential gene analysis. The interested genes were analyzed and obtained using GO and KEGG, and the key genes in the key modules were searched using the Molecular Complexity Module (MCODE) plug-in in Cytoscape. Results: After multi-data set analysis using the data set obtained from THE GEO database, 116 differentially expressed genes, 56 highly expressed genes and 60 low-expressed genes were obtained. After the protein expression network was constructed, the key gene for PTPRB was found out by using the Molecular Complexity Module (MCODE) plug-in in Cytoscape. Conclusion: Bioinformatics analysis of different intracranial aneurysm wall samples shows that PTPRB down-regulation has a high correlation with intracranial aneurysm rupture, which provides a potential alternative target for drug therapy and prevention of intracranial aneurysm rupture.