微小RNA-744在动脉粥样硬化患者中表达的诊断和预后意义评估

Diagnostic and Prognostic Significance of microRNA-744 Expression in Patients with Atherosclerosis

目的:动脉粥样硬化(Atherosclerosis, AS)是一种常见的血管疾病,是冠心病、缺血性心肌病、心力衰竭、血栓栓塞病的病理基础,具有较高的发病率和死亡率。近期大量的研究证明microRNAs (miRNAs)在AS的诊断和预后中发挥着至关重要的作用。本研究通过检测miR-744在AS中的表达情况,进一步评估miR-744对AS诊断和预后价值的影响。方法:采用荧光定量PCR技术检测120例AS患者和58例健康对照人群血清中miR-744的表达情况。通过受试者工作特征曲线(ROC曲线)分析miR-744的诊断的价值。使用Spearman相关系数评估miR-744与颈动脉内膜–中膜厚度(CIMT)的相关性,利用Kaplan-Meier生存曲线和多因素Cox回归分析miR-744在AS中的预后价值。结果:相较于健康对照组,AS患者血清中miR-744的表达水平显著降低(P 【0.001),且miR-744的表达水平与CIMT值呈显著负相关性(r = −0.776, P 【0.001)。ROC曲线的AUC为0.887,特异性为93.33%,敏感性为82.85%,证实miR-744可能是AS的潜在的良好诊断标志物。生存分析表明miR-744低表达的患者发生心血管事件的概率较高(log rank P = 0.005),miR-744可能是AS的潜在预后标志物(HR = 2.680, 95%CI = 1.105~6.503, P = 0.029)。结论:miR-744的低表达可能是良好的AS诊断标志物,并且与AS的不良预后相关。

Objective: Atherosclerosis (AS) is a common vascular disease that is the pathological basis of coronary heart disease, ischemic cardiomyopathy, heart failure, thromboembolic disease, and has high morbidity and mortality. A large number of recent studies have demonstrated that microRNAs (miRNAs) play a crucial role in the diagnosis and prognosis of AS. The aim of this study was to examine the expression level of miR-744 in AS and to evaluate the diagnostic and prognostic value of miR-744 in AS. Methods: The expression of miR-744 in serum of AS patients and healthy control group was detected by fluorescence quantitative PCR. The diagnostic value of miR-744 was analyzed by the ROC curve. Spearman correlation coefficient was used to evaluate the correlation between miR-744 and carotid intima-media thickness (CIMT), and Kaplan-Meier survival curve and Multivariate Cox regression were used to analyze the prognostic value of miR-744 in AS. Results: Compared with the healthy control group, the expression level of miR-744 in the serum of AS patients was significantly reduced (P