MYK对乙酸型胃溃疡大鼠胃组织GAS、SS和血清NO、NOS、EGF的影响

Effect of MYK on Expressions of Gastric Tissue GAS, SS and Serum NO, NOS, EGF in Rat with Gastric Ulcer

目的:将探讨MYK (蒙药验方溃疡散)——治疗胃溃疡民间使用验方药对乙酸型胃溃疡大鼠胃组织GAS (胃泌素)、SS (生长抑素)和血清NO (粘膜保护因子——一氧化氮)、NOS (一氧化氮合酶)、EGF (表皮生长因子)的影响。方法:采用乙酸灼烧法复制大鼠胃溃疡模型,实验分为5组,即模型组、阳性对照组、MYK高剂量给药组、MYK中剂量给药组、MYK低剂量给药组等5组,每组12只连续给药14天后,常规处死,解剖剪取胃部溃疡组织,用ImageJ图像分析法测定胃黏膜溃疡面积并计算胃溃疡抑制率,检测大鼠血清NO、NOS、EGF指标和胃组织GAS和SS的指标以及观察各组间的病理组织特征。结果:MYK对大鼠慢性(乙酸型)胃溃疡具有明显的抑制作用(P 【0.05或P 【0.01),MYK各组大鼠血清NO、NOS、EGF含量增加,与模型组比较均有明显差异(P 【0.05)。MYK降低了大鼠胃组织中的GAS,增高了SS。结论:MYK对乙酸型大鼠胃溃疡具有显著的抑制作用,其作用机制可能与胃组织GAS、SS和血清NO、NOS、EGF有关。

Objective: To investigate the effects of MYK on GAS, SS and serum NO, NOS, EGF in gastric tissue of rats with gastric ulcer. Methods: Rat models of ulcer were established by using Acetic Acid Type of Gastric Ulcer, and rats were randomly divided into 5 groups, named model group, positive control group, MYK high-dose group, MYK medium-dose group, and MYK low-dose group. There were twelve rats in each group which were killed conventionally after 14 days of continuous administration. The gastric ulcer tissue was dissected, and the gastric mucosa ulcer area was determined by ImageJ image analysis method and the gastric ulcer inhibition rate was calculated. Serum NO, NOS and EGF, GAS and SS in gastric tissues were detected, and pathological characteristics of each group were observed. Results: MYK significantly inhibited the acetic acid-type gastric ulcer in rats (P <0.05 or P <0.01). The serum NO, NOS, EGF levels in the MYK high, medium and low dose groups were significantly increased compared with the model group, and there were significant differences (P <0.05). MYK decreased the GAS in the stomach tissue of rats, and increased SS. Conclusion: MYK can significantly inhibit gastric ulcer in acetic acid rats, and its mechanism may be related to gastric tissue GAS, SS and serum NO, NOS, EGF.